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KMID : 1005220070220030149
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Journal of Embryo Transfer
2007 Volume.22 No. 3 p.149 ~ p.154
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Expression of Two-pore Domain K^+ Channels in Endometrial Cells of Korean Cattle
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Kang Da-Won
Kim Eun-Suk
Yang Hye-Young
Choe Chang-Yong
Han Jae-Hee
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Abstract
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Endometrial cells play important roles in implantation and during pregnancy. This study was carried out to identify whether two-pore domain K^+;(K_{2P}) channels are expressed in endometrial cells of Korean cattle. K_{2P} channels set the resting membrane potential of many kinds of neuronal cells in the central and peripheral nervous systems. RT-PCR data showed that TASK-1, TASK-3, TREK-1, TREK-2, and TRAAK were expressed in bovine endometrial cells, and the mRNA expression levels were similar between endometrial cells with or without endometritis. The protein expression was confirmed by using commercially available polyclonal antibodies (TASK-3, TREK-1, TREK-2, and TRAAK). TASK-3 and TREK-1 were expressed in all area of endometrial cells including nuclei, while TREK-2 and TRAAK were expressed in all area of cells except nuclei. These results demonstrate for the first time the presence of K_{2P} channel in endometrial cells of Korean cattle.
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KEYWORD
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two-pore domain K^+ channel, endometrial cell, Korean cattle, endometrium
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